Widefield Viewing Modules for DIY Cerna® Systems

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LAURE1 Trinocular Being Attached to a Cerna Epi-Illumination Module

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Drawing of LAURE1 and LAURE2 Trinoculars

• 10X Eyepieces to Observe FOV with Naked Eye
• Upright-Image Trinoculars
• Available With or Without IR Filters for Eye Protection
• Top-Located Port for Camera Attachment Using Camera Tube
• Adjustable Interpupil Distance
• Male D1N Dovetail on Bottom

These trinoculars are ideal for viewing a sample with the naked eye using the included 10X eyepieces or with a camera connected to the top-located camera port via a camera tube (camera and camera tube sold separately). A lever located on the side of the housing directs the incoming light to either the eyepieces or the camera. 

Each camera port has either a D2N or D5N female dovetail connector that accepts a compatible camera tube, which mechanically positions the camera sensor at the image plane and ensures parfocality with the eyepieces. See the table below for compatible camera tubes. A 2 mm hex key (not included) is used to lock the camera tube in place.

A bottom-located D1N male dovetail connector is provided to attach the trinoculars to other modules such as Cerna microscope bodies, epi-illuminator modules, or double camera ports (available below). For customers interested in constructing a custom system, adapters (available below) that attach directly to the D1N or D2N dovetails on these trinoculars provide compatibility with Thorlabs' 30 mm Cage Systems and SM-Threaded Lens Tubes.

Upright-Image Trinoculars
These LAUREx trinoculars produce an upright image of a sample. The LAURE1 includes a filter before the eyepieces that blocks NIR light. This filter will not block NIR light that is sent to the camera port and it cannot be removed. The LAURE2 does not ship with a blocking filter, but accepts the TF1 NIR blocking filter, available below, with the use of an SM30RR retaining ring (not included).

The drawings for the LAURE1 and LAURE2 trinoculars are shown to the right. The LAUREx trinoculars have an internal TTL200 tube lens. This tube lens has a focal length of 200 mm; see the expandable drawing to the right for the location of the image plane.

These trinoculars incorporate several additional features. A red vibration damping knob on the side of the housing allows the detent mechanism on the carriage slider to be disengaged, which is useful for electrophysiology applications that require minimal vibration when switching between the eyepiece and camera port. They also include a carriage position indicator switch via a 2.5 mm phono jack, allowing users to connect a laser interlock; it is designed to break the circuit in a laser interlock when the carriage is in the eyepiece output position. For custom-built optical detection systems, these trinoculars have 4-40 taps on the top D2N dovetail connector for compatibility with 30 mm cage systems and 4-40 taps on the bottom D1N dovetail for compatibility with 60 mm cage systems.

System Magnification
The total system magnification will be the multiplicative product of the objective magnification and the eyepieces or camera tube magnification, depending on which is being used. To achieve an objective's stated magnification, the objective must have been designed for systems using a 200 mm tube lens. Please note that the eyepieces and camera will see a different FOV due to a difference in magnification in each path. Please see the Magnification & FOV tab for more information on objective's magnification dependance on the system's tube lens and how paths with differing magnifications will see a different FOV.

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Fine Focus Adjuster (Item # SM1ZM) on Camera Tube (Available on Item #'s TC1X, WFA4100, WFA4101, and WFA4102)

• Positions Camera Sensor at Image Plane with 1X, 0.75X, or 0.5X Magnification            
• Versions with Tube Lens Form an Image on a Camera Sensor
• Versions without Tube Lens Provide Optical Distance When Using Trinoculars or Double Camera Ports
• C-Mount (1.00"-32) Threads for Camera Attachment

These camera tubes provide the mechanical spacing necessary to align a camera sensor to the imaging plane in a microscopy system. The top of each camera tube has external C-Mount (1.00"-32) threading that accepts Thorlabs' scientific cameras, as well as cameras from most major manufacturers. In order to balance the size of the field of view (FOV) displayed on the camera against the resolution of the microscope, our camera tubes are offered in several magnifications from 1X to 0.5X. Greater magnification improves the resolution, but it also decreases the size of the FOV. Please see the Magnification & FOV tab for more information.

The WFA4100, WFA4101, and WFA4102 all include either a 200 mm, 150 mm, or 100 mm focal length tube lens, respectively, which allows them to be used in place of trinoculars for applications where the eyepieces are not needed. The tube lens and bottom-located male D1N dovetail make them compatible with the front port of the CSD1001 and CSD1002 double camera ports (available below), microscope bodies, or epi-illuminator modules. To compensate for mechanical tolerances or alignment issues, these camera tubes also include an SM1ZM Fine Focus Adjuster directly before the camera, shown in the photo to the right.

In contrast, the other camera tubes available here do not have built-in tube lenses. They act as a spacer with or without magnification that positions the camera's sensor at the imaging plane when used with the compatible trinocular or double camera port. The lack of a tube lens and the bottom-located male D2N, D2NB, or D5N male dovetail make them compatible with the rear port of the CSD1002 double camera port (available below) or trinoculars (sold above). Additionally, the D2N dovetail on the TC1X allows it to be used with the LCPN6 Camera Tube Adapter (available below) for the construction of custom assemblies. To compensate for small mechanical tolerances or alignment issues, three 5/64" (2 mm) hex setscrews on the side of the housing can be used to adjust the camera position.

Complete compatibility details can be found in the table below. For more information on the dovetails used, please refer to the Microscope Dovetails tab.

Please note that an adjustable C-mount to SM2 camera tube can be constructed using an SM1A9 Adapter, SM1ZM Zoom Housing, SM1L03 Lens Tube, SM1A2 Adapter, and a Ø2" Stackable Lens Tube, which is used as a spacer to compensate for the working distance of the tube lens. For more details on assembling this camera tube, please see the full presentation for our infinity-corrected tube lenses.

Specifications and Compatibility
Item #	Magnificationa,b	Dovetail	Included Tube Lens	Included Camera Focus Adjuster	Compatibility with Trinoculars	Compatibility with Double Camera Ports	Camera Threading
WFA4100	1X	Male D1N	Yes; f = 200 mm	Yes (SM1ZM)	Noc	CSD1001 and CSD1002 Front Port	C-Mount (1.00"-32)
WFA4101	0.75X		Yes; f = 150 mm (AC508-150-A)
WFA4102	0.5X		Yes; f = 100 mm (AC300-100-A)
WFA4112	1X	Male D2NB	No	No	No	CSD1002 Rear Port
TC1X	1X	Male D2N	No	Yes (SM1ZM)	LAURE1 and LAURE2	No

• The magnification values are calculated assuming a design tube lens focal length of 200 mm. See the Magnification & FOV tab for these calculations and a list of design tube lens focal lengths for different manufacturers.
• Greater magnification improves the resolution, but also decreases the size of the FOV. Please see the Magnification & FOV tab for more information about the attainable FOV.
• Aside from the mechanical incompatibility between the dovetail mounting features, the trinoculars above and WFA4100, WFA4101, and WFA4102 Camera Tubes each have a built-in 200 mm, 150 mm, or 100 mm focal length tube lens.

• Replacement Eyepiece for Cerna Trinoculars (Sold Above)
• 10X Magnification
• Field Number: 22 mm

The TE10X eyepiece is identical to the ones included in the LAURE1 and LAURE2 Cerna trinoculars. It offers 10X magnification and has a field number of 22. The eyepiece features an adjustable focus that allows users to rotate the housing while not rotating the optics inside. It can be used with reticles and attached to trinoculars by sliding the narrower, Ø1.18" end of the eyepiece into the eyepiece slot on the trinoculars. Three notches on the housing secure the eyepiece in place once installed.

• IR Filter for Cerna Trinoculars (Sold Above)
• OD_avg > 6 in Rejection Region
• T_avg > 90% in Transmission Region
• 30 mm Outer Diameter

The TF1 IR blocking filter is identical to the one included in the LAURE1 Cerna trinoculars. It can be installed before the eyepieces in the LAURE2 trinoculars (sold above), which do not include a blocking filter, using an SM30RR retaining ring (not included); only one filter is needed for both eyepieces. The filter transmits light from 375 - 650 nm and blocks light from 700 - 1400 nm. It features a durable immersed dielectric coating on a Borofloat^® substrate. 

Note: Borofloat^® is a registered trademark by a company of the Schott group.

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The TF1 filter provides >90% average transmission from 375 to 650 nm, denoted by the blue shaded region. 

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The TF1 filter has an average optical density of >6 from 700 to 1400 nm, denoted by the blue shaded region.

Item #	SM2N2
Microscope Connection	Ø1.18" Eyepiece Tube (Alignment Slot, 5 Places)
SM Threading	Internal SM1 (1.035"-40) External SM2 (2.035"-40)
Cage Compatibility	30 mm Cage System (4-40 Tap, One Side, 4 Places)
Clear Aperture	Ø0.90" (22.9 mm)
Adapter Profile (Click for Drawing)

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Two SM2N2 adapters attached to the trinocular eyepieces.

• Replace Trinocular Eyepiece for DIY Construction
• Compatible with SM1, SM2, and 30 mm Cage Construction Systems

The SM2N2 Eyepiece Adapter allows custom-built optical detection systems to attach to either eyepiece on the trinoculars of a Cerna Microscope. This adapter replaces the lens element on the eyepiece that sets the image plane at the the back of the eyes (see image to the right). Five alignment slots ensure the adapter fits snugly inside the eyepiece without rotation; because of the drop-in nature of this adapter, take care the attached system does not overbalance the 40 g eyepiece adapter when it is inside the trinoculars.

This adapter features internal SM1 (1.035"-40) threading for Ø1" lens tubes; two SM1RR retaining rings are included to secure an optic inside the adapter. The adapter also has external SM2 (2.035"-40) threading for Ø2" lens tubes. The face with the item # engraving has 4-40 tapped holes for 30 mm cage systems.

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Knob on the Front of the CSD1001 Controls Dichroic Mirror to Redirect Light

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Slider on the Side of the CSD1002 Directs Light to One Camera or the Other

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CSD1001 Operation Diagram

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CSD1002 Operation Diagram

• Allows Two Cameras to be Simultaneously Attached to the Microscope
• CSD1002 Double Camera Port:
  • Fixed Magnification
  • All Light can Be Sent to the Front or Rear Port
• CSD1001 Double Camera Port:
  • Variable Magnification Using a Side-Located Knob
  • Light Can be Sent to Front Port or Split with Visible to Front and NIR to Rear

These Double Camera Ports let you simultaneously attach two cameras to a microscope system. The use of two separate cameras, which independently detect the signals from the sample, adds a significant amount of experimental flexibility.

The CSD1002 Double Camera Port provides fixed magnification for the front and rear cameras. As shown in the operation diagram to the right, this camera port contains an internal silver mirror that can be moved into the optical path to reflect all incoming light to the rear port.

In contrast, the CSD1001 Double Camera Port offers fixed magnification for the front camera and variable magnification of 0.35X, 2X, or 4X for the rear camera. The magnification is set by rotating a knob on the right side of the housing. As shown in the operation diagram to the right, it also contains an internal dichroic mirror that can be moved into the optic path to transmit visible light to the front port and reflect NIR light to the rear port. A graph showing the transmission and reflectance information can be found in the table below. The rear port also has a built-in NIR DIC analyzer that can be selectively added into the beam path using a side-located lever.

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LAURE1 Trinoculars Attached to the Front Port of the CSD1001 Double Camera Port

Camera Mounting
The front port of each double camera port contains a female D1N dovetail for mounting widefield assemblies used to view the FOV. As shown in the diagram above, there is no internal tube lens prior to the first port of either system. Therefore, any component mounted to this port requires a tube lens to resolve the image from the objective. To remain parfocal with the rear port, the focal length of the tube lens should be 200 mm. Solutions from Thorlabs' DIY Cerna components include any of the camera tubes with a built-in tube lens or our trinoculars (available above). For more information on how the tube lens focal length affects the system magnification, see the Magnification & FOV tab for details.

In contrast to the front port, an internal tube lens is provided before the rear port of each double camera port. The rear port of the CSD1002 has a D2NB dovetail to attach the WFA4112 1X camera tube (available above). This camera tube is designed to mechanically align a mounted camera with the image plane provided by the internal tube lens and contains no internal optics. The rear port for the CSD1001 contains an externally threaded C-Mount (1.00"-32) for direct attachment of a scientific camera that will be parfocal with a camera mounted to the front port (assuming a 200 mm tube lens was used).

Item #		CSD1002	CSD1001
Top Connection Interface	Front Port	Female D1N	Female D1N
	Rear Port	Female D2NBa	Externally Threaded C-Mount (1.00"-32)b
Bottom Connection Interface		Male D1N	Male D1N
Location of Internal Tube Lens		Before Rear Porta	Before Rear Portb
Reflector for Rear Port		Silver Mirrorc	Dichroic Mirror
Mechanical Drawing (Click for Details)

• The WFA4112 1X Camera Tube is required to align a mounted camera at the image plane set by the internal tube lens. 
• Image plane is located at the flange focal distance of the included C-Mount (1.00"-32) thread on the rear port. No additional components are needed to align a camera to this image plane.
• The CSD1002 has an internal silver mirror that can redirect all of the incoming light to the rear port.

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Mechanical Diagram of the 2CM2 Double Camera Port

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Mechanical Diagram of the 2CM1 Double Camera Port

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Live two-channel composite image generated using 2CM1 Mount, previous-generation scientific CCD cameras, and ThorCam software. The image shows Fluorescence (Pink) and DIC (Grayscale) images of a mouse kidney.

• Microscope Adapters Allow Two Scientific Cameras to Image a Single Optical Input Simultaneously
• Optics Not Included:
  
  • Accepts 25 mm x 36 mm Dichroic Filters or Beamsplitters
  • Accepts Standard Ø25 mm or Ø1" Filters on Outputs
• Fine Pitch Rotation and XY Adjustment for Image Co-Registration
• Coarse Focus Adjustment for Parfocalization of Cameras
• Mechanically Adapt to Most Cerna, Olympus, and Nikon Upright Microscopes Using Thorlabs' Standard SM1 Interface (Microscope Camera Port Adapters Sold Separately)

Thorlabs' two-camera mounts are designed to attach two Thorlabs scientific cameras to a standard microscope, allowing simultaneous imaging of a single optical output. A rotation mount allows for 360° of rotational adjustment (±8° fine adjustment) for the reflected camera while a translation mount gives 4 mm linear XY adjustment of the transmitted camera. Both camera mounts have coarse focus adjustment by manually translating the cameras, allowing for parfocalization of both images. The 2CM1 and 2CM2 mounts have up to 15 mm and 11 mm of adjustment, respectively, using the cage rods, although this adjustment range may be limited by the geometry of the camera's front face.

Each mount includes a DFM1 fluorescence filter cube, which is designed to hold a fluorescence filter set (dichroic mirror, excitation filter, and emission filter) as well as plate beamsplitters or other similarly sized optics. See the table to the right for compatible optic sizes. The filter cube has an insert to hold filter set components with a kinematic design for easy swapping between mounted filter sets without requiring realignment. A DFM1T1 filter cube insert can also be used for mounting additional filter sets. Please note that these mounts do not include tube lenses.

These camera ports are ideal for use with Thorlabs' scientific cameras and ThorCam software. The 2CM1 mount is designed for cameras with 60 mm cage system taps on the front, such as our cooled sCMOS and CMOS cameras, while the 2CM2 mount is identical except for the inclusion of two LCP4S cage size adapters for compatibility with 30 mm cage system taps, such as those found on our compact scientific cameras with sCMOS and CMOS sensors. The ThorCam user interface, provided for free with our scientific cameras, includes a plug-in to allow for multiple live camera images to be overlaid into a real-time 2-channel composite, eliminating the need for frequent updates of a static overlay image. This live imaging method is ideal for applications such as calcium ratio imaging and electrophysiology.

To see example applications where these camera ports are used and how various filters and dichroics are used, please see the full presentation. For double camera ports with an internal tube lens please see the CSD1001 and CSD1002 above.

Microscope Compatibility

The input port of each camera port adapter has external SM1 (1.035"-40) threading and places Thorlabs scientific cameras' sensors at a distance of 4.05" to 4.17" (102.9 mm to 106.0 mm) from the base of the mount (see drawings to the upper right), which may be outside the parfocal distance of some microscopes. See the compatibility information below for details.

Thorlabs' Cerna Microscope Systems
These camera ports can be mounted directly onto our previous-generation inverted-image trinoculars using the SM1A58 adapter (available below). When used with the trinoculars, each mounted camera will be parfocal with the eyepieces. Alternatively, a custom camera tube assembly can also be made to support the two-camera mount. This can be done using the WFA4111 adapter and lens tube or cage system assemblies. When used with upright-image trinocs the camera sensors will not be parfocal with the eyepieces.

Other Commercial Microscopes
Thorlabs offers a line of microscope camera port adapters that will allow these two-camera mounts to be installed on many commercial microscopes. When used with inverted microscopes from Nikon and Olympus the camera sensor will be outside the parfocal distance of the microscope and therefore should only be used if parfocality with the eyepieces is not needed. These mounts will be parfocal with upright microscopes from Nikon and Olympus. For Olympus BX or IX microscopes, Thorlabs offers the SM1A51 camera port adapter with SM1 threading. For upright Nikon Eclipse microscopes, the two-camera mount can be attached to the trinocular's camera port using our SM1A58 camera port adapter.

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CSA3000 Used to Mount a Custom Epi-Illuminator and Widefield Viewing Apparatus with a sCMOS Camera

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CSA3010 Used to Mount a Custom Epi-Illuminator and Widefield Viewing Apparatus with a sCMOS Camera

• Male D1N Dovetail on Bottom for Attachment to DIY Cerna Microscope Bodies
• Available in Two Sizes in Imperial and Metric Versions:
  • Imperial: 14.00" x 11.00" or 18.00" x 4.60"
  • Metric: 350.0 mm x 275.0 mm or 450.0 mm x 116.8 mm
• 1/4"-20 or M6 x 1.0 Mounting Holes

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Each breadboard has a male D1N dovetail on the bottom.

These black-anodized aluminum breadboard tops support user-designed widefield viewing apparatuses, epi-illumination pathways, and laser scanning pathways on top of upright Cerna microscopes. Each contains a Ø1.5" (Ø38.1 mm) through hole that is centered on a male D1N dovetail. This dovetail allows the breadboard to be connected directly to the epi-illumination arm of the microscope body, and it can also be used to stack the breadboard on top of an epi-illumination module. Additional details on the dovetail are available in the Microscope Dovetails tab.

The breadboards are available in two sizes. The larger version [Item # CSA3000(/M)] provides additional work surface, but protrudes past the sides of the epi-illumination arm, which may restrict approach angles around the objective for micromanipulators. The smaller version [Item # CSA3010(/M)] does not restrict approach angles and also has eight 4-40 taps around the Ø1.5" through hole for 30 mm and 60 mm cage systems.

In configurations where the breadboard is mounted directly on top of the epi-illumination arm, four M4 counterbores can be used to provide additional mounting stability.

Item #	CSA3000	CSA3000/M	CSA3010	CSA3010/M
Dimensions (L x W)	14.00" x 11.00"	350.0 mm x 275.0 mm	18.00" x 4.60"	450.0 mm x 116.8 mm
Breadboard Thickness	1/2"	12.7 mm	1/2"	12.7 mm
Hole Size and Spacing	1/4"-20 Tapped Holes on 1" Centers	M6 x 1.0 Tapped Holes on 25 mm Centers	1/4"-20 Tapped Holes on 1" Centers	M6 x 1.0 Tapped Holes on 25 mm Centers
Number of Tapped Holes	154	154	87	89
Cage System Compatibility	-		Four 4-40 Taps for 30 mm Cage Systems Four 4-40 Taps for 60 mm Cage Systems
Click for Mechanical Drawing
Dovetail	Male D1N
Material	Matte Black Anodized Aluminum

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In this setup, the CSA1003 D1N Adapter is connecting lens tube and cage system components to a WFA2002 Epi-Illuminator Module.

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The WFA4111 and SM2A59 adapters allow our infinity-corrected tube lenses to be easily integrated with Cerna microscopes and SM2-threaded components. 

• Extend Versatility of Our Lens Tube and Cage Construction Systems to DIY Cerna Systems
• Compatible with DIY Cerna Modules that Have D1N, D2N, or D2NB Dovetails

These dovetail adapters integrate Thorlabs' Cerna microscopy platform with our SM1 (1.035"-40) lens tube, SM30 (M30.5 x 0.5) lens tube, SM2 (2.035"-40) lens tube, 30 mm cage, and 60 mm cage construction systems. They are ideal for creating custom widefield viewing, epi-illumination, and trans-illumination apparatuses. Additionally, the WFA4111 and SM2A59 adapters feature internal M38 x 0.5 and SM2 threads, respectively, that can directly accept one of our infinity-corrected tube lenses. We also offer the LCPN3 trinocular port adapter, designed to allow Olympus trinoculars that have a male D5Y dovetail to be used with DIY Cerna systems.

See the table below for adapter features and the images to the right for application ideas. Please note the dovetail designations are specific to Thorlabs products; see the Microscope Dovetails tab for details.

Item #	Dovetaila	Threading	Cage Compatibility	Clear Aperture	Adapter Profile (Click for Drawing)
LCPN2	Male D1N	Internal SM30b	30 mm Cage System (4-40 Tapc, 4 Places) 60 mm Cage System (Ø6 mm Bore, 4 Places)	Ø1.10" (27.9 mm)
LCPN3	Male D1N Female D5Y	Internal SM30b	60 mm Cage System (Ø6 mm Bore, 4 Places)	Ø1.10" (27.9 mm)
WFA4111	Male D1N	Internal M38 x 0.5d External SM2	Nonee	Ø1.47" (37.0 mm)
SM2A59	Male D1N	Internal SM2f	Nonee	Ø1.69" (43.0 mm)
LCPN4	Male D1N	Internal SM2g	60 mm Cage System (Ø6 mm Bore, 4 Places)	Ø1.74" (44.3 mm)
CSA1003	Female D1N	None	60 mm Cage System (Ø6 mm Bore, 4 Places)	Ø1.50" (38.1 mm)
SM1A58	Male D2N Male D2NB	Internal SM1f External SM2	30 mm Cage System (4-40 Tap, 4 Places)	Ø1.008" (25.6 mm)
LCPN6	Female D2N	Internal SM1f	30 mm Cage System (4-40 Tapc, 4 Places) 60 mm Cage System (Ø6 mm Bore, 4 Places)	Ø1.01" (25.6 mm)
LCPY3	Female D5Y	None	30 mm Cage System (4-40 Tapc, 4 Places) 60 mm Cage System (Ø6 mm Bore, 4 Places)	Ø1.26" (32.0 mm)

• Additional information on dovetails is available in the Microscope Dovetails tab.
• This internal SM30 threading is compatible with our SM30RR retaining rings. Two SM30RR retaining rings are included.
• These tapped holes are on the side opposite the dovetail only.
• This internal M38 x 0.5 threading is compatible with our SM38RR retaining rings.
• An SM2-threaded cage plate can be used to convert between SM2 lens tubes and 60 mm cage systems.
• This internal threading is not deep enough for mounting optics.
• This internal SM2 threading is compatible with our SM2RR retaining rings. One SM2RR retaining ring is included.